01925 a2200361 4500 1859963609 20250317100401.0 250312042004xx eng 9781859963609 Taylor & Francis GBP 71.99 BB 01 eng PSAK thema PSF thema PSD thema MFK thema PSAK bic PSF bic PSD bic MFK bic SCI010000 bisac SCI029000 bisac SCI049000 bisac SCI086000 bisac 571.61 bisac Jim Mcwhir Gene Targeting and Embryonic Stem Cells 1 Taylor & Francis 20040701 216 p Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference. The recent isolation of human embryonic stem cells and the potential of these cells for therapeutic applications has generated an entirely new methodology. Similarly, gene targeting methodology has recently been extended to nuclear donor cells in ungulate species. This volume will be invaluable for both new and established researchers in the field of human embryonic stem cells, and to biotech companies engaged in the production of transgenic proteins in livestock, xenotransplantation and the development of animal models. Alison Thomson A01 1191 1191