01925    a2200361   4500001001100000005001700011008003900028020001800067037003600085040000700121041000800128072001600136072001500152072001500167072001500182072001400197072001300211072001300224072001300237072002100250072002100271072002100292072002100313072001800334100001500352245004400367250000600411260003100417300001000448520106600458700002401524999001501548185996360920250317100401.0250312042004xx                   eng    a9781859963609  bTaylor & FranciscGBP 71.99fBB  a01  aeng7 aPSAK2thema7 aPSF2thema7 aPSD2thema7 aMFK2thema7 aPSAK2bic7 aPSF2bic7 aPSD2bic7 aMFK2bic7 aSCI0100002bisac7 aSCI0290002bisac7 aSCI0490002bisac7 aSCI0860002bisac7 a571.612bisac1 aJim Mcwhir10aGene Targeting and Embryonic Stem Cells  a1  bTaylor & Francisc20040701  a216 p  bGene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference. The recent isolation of human embryonic stem cells and the potential of these cells for therapeutic applications has generated an entirely new methodology. Similarly, gene targeting methodology has recently been extended to nuclear donor cells in ungulate species. This volume will be invaluable for both new and established researchers in the field of human embryonic stem cells, and to biotech companies engaged in the production of transgenic proteins in livestock, xenotransplantation and the development of animal models.1 aAlison Thomson4A01  c1191d1191