02424 a2200325 4500001001100000005001700011008004200028020001800070037003700088040000700125041000800132072001500140072001500155072001500170072001300185072001300198072001300211072002100224072002100245072002100266072002100287072002000308100002100328245003700349250000600386260002400392300001000416520165700426999001502083142004665920250317100407.0250312042009xx 105 eng  a9781420046656 bTaylor & FranciscGBP 160.00fBB a01 aeng7 aPSB2thema7 aPSF2thema7 aPSD2thema7 aPSB2bic7 aPSF2bic7 aPSD2bic7 aMED0080002bisac7 aSCI0170002bisac7 aSCI0490002bisac7 aSCI0860002bisac7 a572.86362bisac1 aRobert S. Matson10aMicroarray Methods and Protocols a1 bCRC Pressc20090120 a246 p bA Step-by-Step Guide to Present and Future Uses of Microarray Technology Microarray technology continues to evolve, taking on a variety of forms. From the spotting of cDNA and the in situ synthesis of oligonucleotide arrays now come microarrays comprising proteins, carbohydrates, drugs, tissues, and cells. With contributions from microarray experts in both academia and industry, Microarray Methods and Protocols is a turn-by-turn roadmap through the processes necessary to perform a successful microarray experiment. This easy to use book addresses the fundamental aspects of preparing and processing microarrays and bead arrays, labeling, and detection. It also includes a detailed How it Works section that discusses the underlying principles of a number of techniques. Troubleshooting guides offer additional advice for the successful performance of more than 100 protocols in 10 chapters that cover work involving nucleic acids, proteins, carbohydrates, and lectins. --Concise and Well-Organized-- With a focus on the preparation and use of microarrays of biomedical relevance, the text describes a variety of microarray formats useful in the assessment of human disease and in genomic and proteomic research. This authoritative resource provides detailed information regarding sample preparation, labeling, array construction processes, substrate chemistry, array printing, and quality control. Originating with the glass microscope slide and biochip, microarray technology is now pressing onward into the nanotechnology frontier. This book is the all-inclusive manual scientists need to take microarray research to the next level of discovery. c1935d1935