000			01925 a2200361 4500
001			1859963609
005			20250317100401.0
008			250312042004xx eng
020			_a9781859963609
037			_bTaylor & Francis _cGBP 71.99 _fBB
040			_a01
041			_aeng
072	7		_aPSAK _2thema
072	7		_aPSF _2thema
072	7		_aPSD _2thema
072	7		_aMFK _2thema
072	7		_aPSAK _2bic
072	7		_aPSF _2bic
072	7		_aPSD _2bic
072	7		_aMFK _2bic
072	7		_aSCI010000 _2bisac
072	7		_aSCI029000 _2bisac
072	7		_aSCI049000 _2bisac
072	7		_aSCI086000 _2bisac
072	7		_a571.61 _2bisac
100	1		_aJim Mcwhir
245	1	0	_aGene Targeting and Embryonic Stem Cells
250			_a1
260			_bTaylor & Francis _c20040701
300			_a216 p
520			_bGene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference. The recent isolation of human embryonic stem cells and the potential of these cells for therapeutic applications has generated an entirely new methodology. Similarly, gene targeting methodology has recently been extended to nuclear donor cells in ungulate species. This volume will be invaluable for both new and established researchers in the field of human embryonic stem cells, and to biotech companies engaged in the production of transgenic proteins in livestock, xenotransplantation and the development of animal models.
700	1		_aAlison Thomson _4A01
999			_c1191 _d1191