000			02103 a2200289 4500
001			0367263556
005			20250317100353.0
008			250312042021xx eng
020			_a9780367263553
037			_bTaylor & Francis _cGBP 56.99 _fBB
040			_a01
041			_aeng
072	7		_aMX _2thema
072	7		_aMX _2bic
072	7		_aMED000000 _2bisac
072	7		_aMED003000 _2bisac
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072	7		_aMED016000 _2bisac
072	7		_aSCI086000 _2bisac
072	7		_a572.6 _2bisac
100	1		_aRoger L. Lundblad
245	1	0	_aChemical Reagents for Protein Modification _b2nd Edition
250			_a1
260			_bCRC Press _c20210227
300			_a352 p
520			_bFirst published in 1991, Chemical Reagents for Protein Modification, 2nd Edition provides a unique combination of theoretical and practical considerations for the use of chemical reagents for site-specific modification of proteins. The book is divided into three sections, with the first section describing general techniques, including information on the organic chemistry of the various modification reactions; the separation and characterization of site-specific modified proteins, including applications to proteins separated by electrophoresis followed by blotting; the specific chemical cleavage of peptide bonds in proteins; the separation of peptides by high-performance liquid chromatography and electrophoresis; and the use of chemical reagents to assess conformational change in proteins. The second section provides an encyclopedic description of reagents and reactions for the site-specific modification of individual amino acid residues in proteins. The final section presents descriptions of the use of chemical reagents to label biologically significant sites in proteins, including enzyme active sites and the use of covalent cross-linking to measure protein-protein interactions. Particular emphasis is placed on the use of photoaffinity reagents. The book will be an extremely useful research tool for all investigators interested in the solution chemistry of proteins.
999			_c415 _d415