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Gene Targeting and Embryonic Stem Cells (Record no. 1191)

MARC details
000 -LEADER
fixed length control field 01925 a2200361 4500
001 - CONTROL NUMBER
control field 1859963609
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20250317100401.0
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 250312042004xx eng
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 9781859963609
037 ## - SOURCE OF ACQUISITION
Source of stock number/acquisition Taylor & Francis
Terms of availability GBP 71.99
Form of issue BB
040 ## - CATALOGING SOURCE
Original cataloging agency 01
041 ## - LANGUAGE CODE
Language code of text/sound track or separate title eng
072 7# - SUBJECT CATEGORY CODE
Subject category code PSAK
Source thema
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Subject category code PSF
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072 7# - SUBJECT CATEGORY CODE
Subject category code PSD
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072 7# - SUBJECT CATEGORY CODE
Subject category code MFK
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072 7# - SUBJECT CATEGORY CODE
Subject category code PSAK
Source bic
072 7# - SUBJECT CATEGORY CODE
Subject category code PSF
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072 7# - SUBJECT CATEGORY CODE
Subject category code PSD
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Subject category code MFK
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072 7# - SUBJECT CATEGORY CODE
Subject category code SCI010000
Source bisac
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Subject category code SCI029000
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Subject category code SCI049000
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072 7# - SUBJECT CATEGORY CODE
Subject category code SCI086000
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072 7# - SUBJECT CATEGORY CODE
Subject category code 571.61
Source bisac
100 1# - MAIN ENTRY--PERSONAL NAME
Personal name Jim Mcwhir
245 10 - TITLE STATEMENT
Title Gene Targeting and Embryonic Stem Cells
250 ## - EDITION STATEMENT
Edition statement 1
260 ## - PUBLICATION, DISTRIBUTION, ETC.
Name of publisher, distributor, etc. Taylor & Francis
Date of publication, distribution, etc. 20040701
300 ## - PHYSICAL DESCRIPTION
Extent 216 p
520 ## - SUMMARY, ETC.
Expansion of summary note Gene Targeting and Embryonic Stem Cells is a practical guide designed for the rapidly growing number of researchers who are moving into this field. Provides details on how to culture, transfect and differentiate established cell lines, and how to isolate new cell lines. Gene targeting experiments are described for a number of cell types, including ungulate fetal fibroblasts, murine ES cells, human embryonal carinoma cells and human ES cells, and include protocols for gene-targeting vectors, DNA transfection and RNA interference. The recent isolation of human embryonic stem cells and the potential of these cells for therapeutic applications has generated an entirely new methodology. Similarly, gene targeting methodology has recently been extended to nuclear donor cells in ungulate species. This volume will be invaluable for both new and established researchers in the field of human embryonic stem cells, and to biotech companies engaged in the production of transgenic proteins in livestock, xenotransplantation and the development of animal models.
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Alison Thomson
Relationship A01

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